new balance outlet Recombinant Mouse Endostatin cl

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Endostatin gene in mice and identification of recombinant clone

Vascular endothelial cells and its mechanism [J]. Fudan University (Medical Sciences), 2005,32 (3) :263-265,[link widoczny dla zalogowanych], 283 (Received :2006 -11-02 Revised :2006-12-27) (Continued from page 85) it was discovered that some endogenous angiogenesis inhibitors, such as thrombospondin, platelet factor 4, prolactin N. Off the end pieces, such as a fragment of prothrombin. Folkman laboratory which discovered Endostatin is particularly important. 1997. OReilly mouth endothelial cells from rat tumor cell conditioned medium was isolated and purified a new protein. Is the end of collagen X Ⅷ 20KD protein degradation products, named Endostatin. Endostatin direct target is the new role of vascular endothelial cells. On the other cells and normal non-static endothelial cell inhibition. OReilly other 【2] confirmed when the mice injected amount of recombinant Endostatin,[link widoczny dla zalogowanych], mouse Lewis lung carcinoma, T241 fibrosarcoma and Emoa transfer of tumor vascular endothelial cells almost completely suppressed, and in the treatment of any side effects not found to show its presence in the field of cancer treatment attractive prospect 【3]. The inhibition mechanism is unclear. xu,[link widoczny dla zalogowanych], etc.] The cell cycle distribution by flow cytometry and found that the main cause endothelial cells Endostatin G. / G capture,[link widoczny dla zalogowanych], S cells decreased. Endostatin generated by the hydrolysis of collagen X Ⅷ. But its enzymatic substrate, are not readily available, limiting the commercialization of natural Endostatin,[link widoczny dla zalogowanych], people hope genetic engineering products. In this study, mice with Trizol extracted total liver RNA. RT. Endostatin gene was amplified by PCR method. Endostatin connected by connecting the reaction to the cloning vector pGEM-4Z, the recombinant pGEM-4Z. Endo transfection of competent E. coliDI-ISct bacteria. A large number of amplified and purified pGEM-4Z. Endo, by PCR, restriction enzyme digestion and sequencing methods to confirm Endostatin successfully constructed the recombinant clone. Endostatin we can further study the gene inserted into expression vector orientation. Into host bacteria, the expression of the required large number of En. dostatin protein. This research is genetic engineering technology to produce En. dostatin provide preliminary experimental evidence.
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