nike sale HepG2 resistant ribosome display antibod

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HepG2 resistant ribosome display antibody library

. (2) The test cells from mice immunized with HepG2 total RNA was amplified spleen antibody variable region fragment against HepG2 cells was constructed ribosome display single chain antibody library constructed with in vitro transcription and translation VH/k5 side must 17 promoter,[link widoczny dla zalogowanych], Kozak sequence and ATG initiation codon, 3 end with ck as a spacer. Because ribosome display template VH/k3 terminal deletion of the stop codon,[link widoczny dla zalogowanych], so that no protein with the ribosome after separation,[link widoczny dla zalogowanych], the formation of single chain antibodies. A mRNA ribosomal ternary complex,[link widoczny dla zalogowanych], which can be easily carried out in vitro selection. (3) VH and VL by a linker peptide between the connection, the study used a design l5 Huston and other _7 peptide linker (GlyaSer) 3, by x-ray diffraction analysis, the connection will not affect the secondary structure of scFv, help restore the natural binding scFv, has good stability and vitality _8I9]. The trial successfully constructed mouse anti HepG2 ribosome display antibody library for screening to obtain specific anti-surface antigen HepG2 laid the foundation for a single-chain antibody.
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