tory burch boots Ginkgo flavonoids (GBE) in the de

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Ginkgo flavonoids (GBE) in the detection and purification of

3.1 The first process to explore a better selection process water back extraction process 311 dry Ginkgo biloba 10 broken a moderate water (leaf weight 20 times) for a 90 ℃ water bath for a filter of a filter seep through a concentrated 4h 95 ℃ for a centrifugation to mention a crude residue 3.1.2 Note the degree of a matte test petroleum ether, ethanol extract of Ginkgo biloba dry process 10_ + broken back of a petroleum ether extract (leaf weight 6-8 times, 15h, 80 ℃ Bu groan a spike filter the main insect residue water soaked _ ÷ _ + _ + over ear of a residue fluid extract alcohol reflux filtrate _ + spike had a spike of a combined filtrate recovered alcoholic crude extract of a yellow heir warm luminosity of a thorn ethanol extraction residue 313 Process of dry Ginkgo biloba extract 10g of alcohol a broken one (70 ℃, 4h) a residue of a filtrate of a filter for a recovery of alcohol {60 ℃, the amount of water fill g) - + _ + flavone crude extract 3.14 absorbance measurement results the extraction process and the analysis of different measurement results in Table 2. Table 2 shows the process of flooding the absorbance values of the reference yield is low, and extract spoilage when stored, taking into account the yield, melting; f at recovery, ease of operation and the rationality of industrial production, the election ethanol extraction process set to continue testing. 3.2 Selection and determination of process parameters for the system test process of ethanol extraction parameters on the results of extraction, according to available information and the actual situation, the choice of ethanol concentration, extraction temperature, extraction and ethanol with the amount of time as a reference factor to determine the total flavonoid content in extracts of the reference index, the orthogonal test. Ginkgo biloba dry each 10g, with a spectrophotometer the absorbance of crude filtrate, the results shown in Table 4. Table 3 solid exemplar of 4 peptide levels and results of orthogonal design table 11111760.12565916521222 ∞ O1439835563133372o254l2o. 83984212387o184107.6895223152O. Zha 77.644.862312550.321115 deleted 73132870l26764O658322379o165 goose 71/529331l27o2284082Oll2851l19 Bo 0128791843s74U monkey 59I1264. Rice 6 o194 lizard lynx spider 8647 18276.9172274 farming l317234 by the orthogonal table analysis, the best combination of factors 4 B,[link widoczny dla zalogowanych], CD3, which uses 40 times the weight of raw materials, 70% ethanol solution at 70-75 ℃ condition immersion mention 6h, the highest leaching rate of 4 flavonoids purified extracts of ginkgo biloba and alcohol treatment thousand dry extract in addition to containing flavonoids and lactone, but also a certain amount of impurities, mainly protein, carbohydrate, high- tannin polymers and molecular proanthocyanidins,[link widoczny dla zalogowanych], which does not remove the impurities will affect the purity of the product. The test extracts with flocculant settling, centrifugation, adsorption and desorption methods such as resin handle, and then by concentration and drying the finished products: the aforementioned method used for treatment of 41 sedimentation containing flavonoids extracted as 2.556% of the crude filtrate, adjust pH 5. O, the amount of added diatomite was 0.25g/lg 'OmL, mixing evenly. Standing 45rain4.2 centrifuge allowed by the flocculation and sedimentation of the extract into a centrifuge tube were centrifuged, the processing time 20rain,[link widoczny dla zalogowanych], the material down to settle down in flocculation, to improve the content of flavonoids GBE. 4.3 After the separation of the purified liquid pumping put too much on the D101 macroporous resin column, the three times after adsorption, liquid color is light to complete the adsorption process. Resin by water washing to further remove impurities, with 70% alcohol solution for desorption,[link widoczny dla zalogowanych], desorption solution obtained. Desorption solution was recovered and the concentration of ethanol,[link widoczny dla zalogowanych], collected in a blast inside the oven. Dried at 60 ℃ for about 3h to get GBE products. The test results showed that the purification of total flavonoids can GBE from 14% to 18% to 24% a 29%. Products out rate of more than 3.0%. 5 Results Cambodian language of the trial process in the extraction of flavonoids in the study. Work better, GBE products from ideal. Using this method of hawthorn leaves, lotus leaves, tea and other herbs were made by dipping a large number of experimental data, the future development of its products in some more in-depth work.
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