nike sale Synchronization PCR technique in plant m

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Synchronization PCR technique in plant molecular quantification of nucleic acid

etersB,[link widoczny dla zalogowanych], JaegerV, RoessnerA. 2001. AnalysisofhumantelomeraserevePsetranscriptasemRNA (h1ERT) expressioninmyxoidliposarcomasusingLightCyelerrea1. . timequantitativerevePsetranscripase-poly. . merasechainreaction. Electrophoresis. 22:1098-1101. SniderJV. Ⅵ rechserMA. LassosIS. 2001. Humandiseasechar-aetefization: real-timequantitativePCRanalysisofgeneex-pression. DrugDiscovToday. 6:1062-1067. TyagiS. KranqerFR. 1996. Molecularbeacons: probesthatfluo-resceuponhybridization. NatBiotechno1.14 :303-308. VandenbreuekeII, VandesompeleJ, PaepeAD,[link widoczny dla zalogowanych], MessiaenL. 2001. QuantificationofsnIieevariantsusingreal-timePCR. NucleicAcidsRes. 29: E68-78. WeisJH, TanSS, MartinBK,[link widoczny dla zalogowanych], WittwerCT. 1992. DetectionofraremRNAsviaquantitativeRT-PCR. TrendsGenet. 8:263-264. WittwerCT. HerrmannMG,[link widoczny dla zalogowanych], MossAA, RasmussenRP. 199r7. ContinuousfluorescencemonitoringofrapidcycleDNAampli-fication. BioTechniques ,22:130-138. (Managingeditors: BAIYu-Hong, ZHAOLi-Hui) synchronization PCR technique in plant nucleic acid quantification of LIU Jin-Yuan (Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China) Abstract: Synchronous PCR is a set of biochemical, optical, and computer technology in one of the closed DNA amplification system that uses fluorescent dyes to combine amplification and detection process, realized in the process of online display PCR PCR reaction, by detecting the fluorescence intensity to absolute quantification of initial template copy number. This technology greatly simplifies and accelerates the process of quantitative nucleic acid, not only rapid, sensitive, accurate, reproducible, and easy to calculate the sample nucleic acid molecules in the absolute starting copy number. The same microarray and other molecular biology techniques with synchronous technology will be in the functional gene PCR analysis of molecular diagnosis and disease play an important role. This review describes synchronization in addition to principles and applications of PCR technology, but also introduced the quantitative Arabidopsis A ~ / IAA gene transcription experiments, and to synchronize operation of the process of PCR were discussed. Key words: synchronous PCR technology; plant nucleic acid quantification; gene expression; disease molecular diagnostics CLC: Q943 Document code: A Article ID :0577 -7496 (2003) 06-0631-07 Received Date :2002-05 -17 Received :2002 -09-06 Foundation item: National Natural Science Foundation of China (3977Oo78; 39780030; 30170080); National High Technology Research and Development Program \.) * Tel :010-62772243; E-mail: . (Editor: the white feather of red, Zhao Lihui)
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