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Anti-infectious bursal disease virus in and of the establishment and application of monoclonal antibodies

Application and construction of the body 83 and Table 3 double antibody EL1SA and summary of AGP review 41BDVTable3Detectionot1BDVbyAGPanddoubleantibodysandwichELISA3 r1) calls the chicken virus in the form of isolated organs bar knot J,[link widoczny dla zalogowanych], and other aspects of pathogenicity of IBDV is very similar to ~, the edge type is acid Double-like RNA, but two small bands of nucleic acids with celery, trapped the acid with SDS-PAGE electrophoresis edge made of two different viruses. H-is a very reliable method. , (2) clear hybridoma antibody is monoclonal antibody screening method exploits or construction and Ju important criteria of quality ring. ELISA as a specific, rapid and sensitive detection method for McAb employed but generally this method of blue screening McAb specific characteristics, especially on the hemagglutination} u in particular in the identification and characterization. Qin poison pen for immunization with the bursal tissue source, cell adapted virus as the ELISA coating antigen, avoiding the factors of pure antigen. VNT is a classic,[link widoczny dla zalogowanych], reliable,[link widoczny dla zalogowanych], very Ding convincing experimental method,[link widoczny dla zalogowanych], but the operation DW trivial, and requires strict sterile. VNT for screening McAb, is not yet even been reported. Pen has been selected by VNT 6 with neutralizing anti-IBDV monoclonal antibody. Neutralizing monoclonal antibody treatment in medical divination has been confirmed ll ', because it is cited the decision of a single cluster of homogeneous antibodies,[link widoczny dla zalogowanych], hardship this can be used for structural analysis of viral antigen and with DNA recombinant technology, basic difficulties Fortunately, at each project to promote vaccine research (3) Bursa IBDV organ is the target organ invasion. . First, the toxic King high. ��N state base, higher levels of renal drug homes. Heart and lung care drug lowest. The results show that the double-antibody ELISA. IBDV cytotoxic drug prices significantly lower than tissue toxicity.
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