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Cadmium - metallothionein antibody and enzyme-linked immunosorbent assay

naldJ. andGarveyjs. Productionandstudyofantibodyproducedagainstratcadmium-thionein. 1mmunochemistry1978; l5: 8577.1ldaM. M. RobertM. BandMichaelPW. MTgeneexpres-sionintesticularinterstiticalcelIsandliverofrattreatedwithcadmium. Toxicology1996; 107:1218. LouisESandCurtisDK. KidneysysthesizeslessMTthanliverinresponsetocadmiumchlorideandcadmium-MT. ToxicologyandAppliedPharmacology1988; 92:95 (1997-05.30 Received Revised Meng Qingyun editor 199,707.02) in blood by gas chromatography PCE Department of Forensic Medicine of Shanxi Medical University (Taiyuan 030001) Preservice Health Institute staff Keming He cardiovascular disease in Shanxi Province Juan,, ■, I__ · a 』PCE (tetrachloroethylene) as a colorless non-flammable and do not dissolve in liquid water is the main component of dry cleaning agent,[link widoczny dla zalogowanych], the human body have a certain toxicity,[link widoczny dla zalogowanych], chronic poisoning can cause central nervous system function nervous system depression, dizziness, drowsiness, nausea and other symptoms, severe acute poisoning can be deadly. Are reported in the literature. By gas chromatography mass spectrometry analysis of blood and tissues of PCE, but the method is complicated and expensive equipment, and carry out certain difficulties. The analytical method fast, simple, sensitive and suitable to do analysis of large numbers of samples rapidly. Are as follows. Materials and Methods (1) reagents and drugs: PCE (Tianjin Chemical Reagent Factory produced) of analytical grade. (2) Experimental animals and exposure methods: four rabbits, weight 2.5 ± 0.5kg (provided by the Experimental Animal Center of the University.) Fasted overnight before the experiment, the PCE air concentration 40mg / I static type exposure 20min, immediately after surgery at different times of carotid artery blood (heparin anticoagulant). (3) Instruments and chromatographic conditions: GC-9A gas chromatograph, PEG20M capillary column 25m × 0.25mm, the column temperature was 90 ℃, into the foreign port temperature 120 ℃, detector FID, N250ml/min, H240ml/min. Air300ml/min. Recorder paper speed 5mm/min,[link widoczny dla zalogowanych], minimum peak area 100. (4) sample handling: take Penicillium Sok wash bottle sealed with a syringe 3ml distilled water and then injected into 2ml of whole blood containing PCE, shake home heating oven 70 ℃ 30min, extracted headspace 100 ~ 1 gas injection. Results (1) chromatography Graphics: Graphics good chromatography, no impurity peak interference, retention time 3.55min. (2) working curves, linear range and detection limit: 5 to take clean penicillin bottles were numbered 1,[link widoczny dla zalogowanych],2,3,4,5 number. Distilled water per tube 3m1. Then add 2ml blank whole blood, followed by adding 0.2,0.4 l, 0.8tal, lbtl, 2pl PCE solution, according to the method of sample treatment injection to the peak area of the PCE concentration plot, the The linear range was 0.02 ~ 1.6mg/ml, the regression equation Y = 70624.1X a 185.5, r: 0.997. The detection limit was O. A pair of mg / ml. (3) to avoid blood poisoning at home PCE determination results table. Schedule of PCE in blood in rabbits poisoned with determination results 【± S. rag / rolln = 4 results show that the headspace gas chromatography method for the determination of PCE in blood fast, simple and sensitive for clinical analysis and emergency poisoning the blood of four dry cleaning employees in the determination of nitrogen to mention the reference of ethylene, but also further toxicological studies of PCE basis. Rabbits poisoned blood through the determination of PCE, the results show that the elimination of PCE in the body faster. Because PCE is gas inhalation, and does not dissolve in water, the elimination method may be excluded from the main airway. (1997-06,[link widoczny dla zalogowanych],28 Received Revised Caitian De 1997-08.28 edit)
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